Given the importance of integrating functional studies – both in vitro and cell-based – into structural biology work, the Structural Biology Initiative includes equipment for a range of such studies. The below instrumentation is available as of April 2016.

For further information, please contact Diane Beckford at diane.beckford@asrc.cuny.edu or at 212-413-3221.

  • Cell-based assays: GE Cytell Automated Imaging System

    GE Cytell Automated Imaging System

    Simple automated cell microscopy instrument, allowing the straightforward collection of multicolor cellular images along with a number of cell-based assays with fluorescent or visible readouts, including assays of cell viability, cell cycle staging, gene expression and others.

  • Phosphoimager: GE Typhoon FLA 9500

    GE Typhoon FLA 9500

    Designed to provide quantitative imaging of gels, blots and other two-dimensional formats of radiolabeled (32P) and fluorescently-labeled samples.

  • Gel imager: GE AI600 RGB Gel Imaging System

    GE AI600 RGB Gel Imaging System

    Used to image DNA gels (UV) and Western blots (chemiluminescence), but can image fluorescence as well.

  • Plate reader for biochemical and cellular assays: Molecular Devices SpectraMax i3

    Molecular Devices SpectraMax i3

    Supports a variety of plate-based biochemical assays, including those utilizing changes in absorbance, fluorescence, or luminescence. Additional capabilities to monitor changes in fluorescence polarization (FP) and AlphaScreen type assays are also equipped.

  • Cell culture facility

    Two cell culture hoods plus four CO2 incubators, microscopes and centrifuges are available for use. Blue light illumination systems are also available for optogenetic control of gene transcription and other cellular properties.