Structural Biology Initiative, Fall 2018

Structural Biology Initiative | Fall 2018

Recent Publications

I. Dikiy, U. R. Edupuganti, R. R. Abzalimov, P. P. Borbat, M. Srivastava, J. H. Freed, K. H. Gardner. (2019) Insights into histidine kinase activation mechanisms from the monomeric blue light sensor. EL346. Proc. Natl. Acad. Sci., DOI: 10.1073/pnas.1813586116.

I. Dikiy, L. Clark, K. H. Gardner, D. M. Rosenbaum. (2019) Isotopic labeling of integral membrane proteins for study by solution NMR relaxation methodsMethods Enzymol., 614: 37-65.

J. Chase, A. Catalano, A. J. Noble, E. T. Eng, P. D. Olinares, K. Molloy, D. Pakotiprapha, M. Samuels, B. Chait, A. des Georges, D. Jeruzalmi. (2018) Mechanisms of opening and closing of the bacterial replicative helicase. eLife, 7, e41140. DOI: 10.7554/eLife.41140

L. Clark, I. Dikiy, D. M. Rosenbaum, K. H. Gardner (2018) On the use of Pichia pastoris for isotopic labeling of human GPCRs for NMR studies. J. Biomol. NMR, 71: 203-211.

A. Dashti, D.B. Hail, G. Mashayekhi, P. Schwander, A. des Georges, J. Frank, A. Ourmazd (co-corresponding author) (2018) Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots. bioRxiv: 291922 (pre-print)

S.T. Glantz, E.E. Berlew, Z. Jaber, B.S. Schuster, K. H. Gardner, B.Y. Chow (2018) Directly light-regulated binding of RGS-LOV photoreceptors to anionic membrane phospholipids.  Proc. Natl. Acad. Sci., 115: E7720-E7727

G. Santulli, D. Lewis, A. des Georges, A. R. Marks, J. Frank (2018). Ryanodine receptor structure and function in health and disease. In Membrane Protein Complexes: Structure and Function (pp. 329-352). Springer, Singapore. DOI: 10.1007/978-981-10-7757-9_11

A. Losi, K. H. Gardner, A. Möglich (2018)  Blue-light receptors for optogenetics.  Chem. Rev.  DOI: 10.1021/acs.chemrev.8b00163

A. J. Noble, V. P. Dandey, H. Wei, J. Brasch, J. Chase, P. Acharya, G. Scarpin M. Rapp, E. T. Eg, W. J. Rice, A. Cheng, C. Negro, L. Shapiro, P.D. Kwong, D. Jeruzalmi, A. des Georges, C. Potter, B. Carragher (2018). Routine single particle CryoEM sample and grid characterization by tomographyeLife7, e34257. DOI: 10.7554/eLife.34257

D.A. Keedy*, Z.B. Hill*, J.T. Biel, E. Kang, T.J. Rettenmaier, J. Brandao-Neto, N.M. Pearce, F. von Delft, J.A. Wells, J.S. Fraser (* contributed equally). (2018) An expanded allosteric network in PTP1B by multitemperature crystallography, fragment screening, and covalent tethering. eLife. DOI: 10.7554/eLife.36307.

L. Calisti, M.C. Trabuco, A. Boffi, C. Testi, L.C. Montemiglio, A. des Georges, et al. (2018) Engineered ferritin for lanthanide binding. PLoS ONE 13(8): e0201859. DOI: 10.1371/journal.pone.0201859

B. B. Minsky, R. R. Abzalimov, C. Niu, Y. Zhao, Z. Kirsch, P. L. Dubin, S. N. Savinov, I. A. Kaltashov (2018) Mass spectrometry reveals a multi-faceted role of glycosaminoglycan chains in factor Xa inactivation by antithrombinBiochemistry. DOI: 10.1021/acs.biochem.8b00199.

B. Johnson (2018) From Raw Data to Protein Backbone: Chemical Shifts Using NMRFx Processing and NMRViewJ Analysis. Methods in Molecular Biology.  DOI: 10.1007/978-1-4939-7386-6_13

L. Clark, I. Dikiy, K. Chapman, K. E. Rödström, J. Aramini, M. LeVine, G. Khelashvili, S. G. F. Rasmussen, K. H. Gardner, D. M. Rosenbaum (2017) Ligand modulation of sidechain dynamics in a wild-type human GPCR. eLife.  DOI: 10.7554/eLife.28505

A. Reade, L. B. Motta-Mena, K. H. Gardner, D. Y. Stanier, O. D. Weiner, S. Woo (2017) TAEL: A zebrafish-optimized optogenetic gene expression system with fine spatial and temporal control. Development. DOI: 10.1242/dev.139238